انبرک های نوری- روشها و پروتکل ها Optical Tweezers_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi PART I HISTORIC VIEWS ON THE INVENTION OF LASERS AND OPTICAL TWEEZERS 1 The Invention of the Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Nick Taylor 2 Art Ashkin and the Origins of Optical Trapping and Particle Manipulation . . . . 11 Matthias D. Koch and Joshua W. Shaevitz PART II TECHNICAL ADVANCES 3 Synthesis of Germanium Nanospheres as High-Precision Optical Tweezers Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Swathi Sudhakar, Pasupathi Rajendran, and Erik Schaffer € 4 Angular Optical Trapping to Directly Measure DNA Torsional Mechanics . . . . . 37 Xiang Gao, James T. Inman, and Michelle D. Wang 5 Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 Anna E. C. Meijering, Julia A. M. Bakx, Tianlong Man, Iddo Heller, Gijs J. L. Wuite, and Erwin J. G. Peterman 6 One-Dimensional STED Microscopy in Optical Tweezers . . . . . . . . . . . . . . . . . . . 101 Tianlong Man, Joost J. Geldhof, Erwin J. G. Peterman, Gijs J. L. Wuite, and Iddo Heller 7 Temperature Quantification and Temperature Control in Optical Tweezers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123 Joost J. Geldhof, Agata M. Malinowska, Gijs J. L. Wuite, Erwin J. G. Peterman, and Iddo Heller 8 High-Resolution Optical Tweezers Combined with Multicolor Single-Molecule Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 Rajeev Yadav, Kasun B. Senanayake, and Matthew J. Comstock PART III DNA, DNA MOTORS AND DNA–PROTEIN INTERACTIONS 9 Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 243 Graeme A. King, Dian Spakman, Erwin J. G. Peterman, and Gijs J. L. Wuite vii10 Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 273 Arnulf M. K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, and Thomas T. Perkins 11 Probing the Interaction Between Chromatin and Chromatin-Associated Complexes with Optical Tweezers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313 Rachel Leicher and Shixin Liu 12 Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 329 Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, and Bo Sun 13 CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, and David S. Rueda PART IV PROTEIN (UN)FOLDING 14 Cotemporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation. . . . . . . . . . . . . . . . . . . . 381 Varsha P. Desai, Filipp Frank, and Carlos J. Bustamante 15 Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 401 Katarzyna Tych and Matthias Rief 16 Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 427 Kevin Maciuba and Christian M. Kaiser 17 Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 461 Huaizhou Jin, Jinghua Ge, Jie Yang, and Yongli Zhang 18 Using Optical Tweezers to Monitor Allosteric Signals Through Changes in Folding Energy Landscapes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 483 Lihui Bai, Mira Malmosi, Lydia Good, and Rodrigo A. Maillard PART V CYTOSKELETAL MOTORS AND PROTEINS 19 High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 513 Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, and Marco Capitanio 20 Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 559 Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap viii Contents21 Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 585 Lu Rao and Arne Gennerich 22 Ultrafast Force-Clamp Spectroscopy of Microtubule-Binding Proteins . . . . . . . . 609 Suvranta K. Tripathy, Vladimir M. Demidov, Ivan V. Gonchar, Shaowen Wu, Fazly I. Ataullakhanov, and Ekaterina L. Grishchuk PART VI FUNCTION AND MECHANICAL PROPERTIES OF FILAMENTS 23 Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 653 Lucas E. Murray, Haein Kim, Luke M. Rice, and Charles L. Asbury 24 Mechanics of Single Vimentin Intermediate Filaments Under Load . . . . . . . . . . . 677 Anna V. Schepers, Julia Kraxner, Charlotta Lorenz, and Sarah Ko ¨ster 25 Quantifying the Interaction Strength Between Biopolymers. . . . . . . . . . . . . . . . . . 701 Charlotta Lorenz, Anna V. Schepers, and Sarah Ko ¨ster PART VII MECHANOSENSING OF MEMBRANE CHANNELS 26 Measuring αβ T-Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging. . . . . . . . . . . . . . . . . . . . . . . . . . . . 727 Hannah M. Stephens, Kristine N. Brazin, Robert J. Mallis, Yinnian Feng, Debasis Banik, Ellis L. Reinherz, and Matthew J. Lang Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 755   مشخصات فایل
عنوان (Title):	Optical Tweezers_ Methods and Protocols
نام فایل (File name):	915-www.GeneProtocols.ir-Optical Tweezers_ Methods and Protocols-Humana Press (2022).pdf
عنوان فارسی (Title in Persian):	انبرک های نوری- روشها و پروتکل ها
ایجاد کننده:	Arne Gennerich
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071622285, 9781071622285
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	35.9 مگابایت
تعداد صفحات (Book length in pages):	753

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