ایمونوهیستوشیمی انتقال سیگنال _ روش ها و پروتکل ها Signal Transduction Immunohistochemistry_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . ix 1 Top Five Considerations When Choosing an Antibody . . . . . . . 1 Amy Archuleta and Michael D. Browning 2 Validating Antibody Specificities for Immunohistochemistry by Protein Blotting. . . . . . . . . 21 Biji T. Kurien and R. Hal Scofield 3 Recent Advances in Chromogens for Immunohistochemistry. . . . . . . 35 Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key 4 Overview of Imaging Techniques in Immunohistochemistry . . . . . . . 51 Gerald Sedgewick 5 Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells . . . . . . . . . . . 83 Pravin J. Mishra and Debabrata Banerjee 6 Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments . . . . . . . . 93 Hemant K. Mishra 7 Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining . . . . . . . . . 113 Jingwen Niu, Jodi Hagen, Feifan Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas 8 Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species. . . . . . . . . . . 127 Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz 9 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes. . . . . . . . . . 143 Sharla L. White, Amy T. Lam, and Hannah D. Buck 10 Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research . . . . . . . . . 163 Simeon C. Daeschler and Gregory H. Borschel 11 Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach . . . . 171 Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, and Bartosz A. Grzybowski 12 Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing . . . . . . . . . . 197 Christine Goetz, Christopher Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier vii viii Contents 13 In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-detection of Phosphorylated EGFR in A431 Cultured Cells . . . . 213 Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny 14 In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue. . . . . . . . . . . 221 Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny 15 Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry . . . . . . . . . . 233 Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova 16 Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells . . . . . . . . 245 Junjiao Wu and Yu Tang 17 Immunohistochemical Techniques for Phosphoproteins . . . . . . 259 Berenice L. Sanz Ressel and Alfredo A. Molinolo 18 Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells . . . . . 265 Laurie S. Minamide, Ryan Hylton, Matthew Swulius, and James R. Bamburg 19 Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Formalin-Fixed Paraffin-Embedded Human Brain Sections. . . . . . . . . . . 283 Clara Mun˜oz-Castro, Ayush Noori, Bradley T. Hyman, and Alberto Serrano-Pozo 20 Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry. . . . . . . . . . 307 Lokman Pang, Matthias Ernst, and Jennifer Huynh 21 Highly Multiplexed Immunofluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometry™. . . . . 317 Thomas D. Campbell, Arne Christians, and Madison A. Tyler Index . . . . . . . . . . . . . . . 323      مشخصات فایل
عنوان (Title):	Signal Transduction Immunohistochemistry_ Methods and Protocols
نام فایل (File name):	985-www.GeneProtocols.ir-Signal Transduction Immunohistochemistry_ Methods and Protocols-Humana Press (2023).pdf
عنوان فارسی (Title in Persian):	ایمونوهیستوشیمی انتقال سیگنال _ روش ها و پروتکل ها
ایجاد کننده:	Alexander E. Kalyuzhny
زبان (Language):	انگلیسی English
سال انتشار:	2023
شابک ISBN:	9781071628102
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	13.7 مگابایت
تعداد صفحات (Book length in pages):	333

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