بیان هترولوگ پروتئین های غشایی- روشها و پروتکل ها Heterologous Expression of Membrane Proteins_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . . . . . . vii Contributors. . . . . . . . . . . . . . . . . . . . xiii 1 Solubilization and Purification of α5β1 Integrin from Rat Liver for Reconstitution into Nanodiscs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Estelle Dransart, Aure´lie Di Cicco, Ahmed El Marjou, Daniel Le´vy, Staffan Johansson, Ludger Johannes, and Massiullah Shafaq-Zadah 2 Membrane Protein Production in Escherichia coli: Protocols and Rules . . . . . . . . 19 Jordi Royes, Pauline Talbot, Christel Le Bon, Karine Moncoq, Marc Uzan, Francesca Zito, and Bruno Miroux 3 Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Margot Di Cesare, Aissatou Maty Diagne, Benjamin Bourgey, Jean-Michel Jault, and Ce´dric Orelle 4 Heterologous Expression of Membrane Proteins in E. coli . . . . . . . . . . . . . . . . . . . 59 Peer Depping, Marı´a Monserrat Roma´n Lara, Athanasios Kesidis, Roslyn M. Bill, Alice J. Rothnie, Douglas F. Browning, and Alan D. Goddard 5 Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 Alexia Gobet, Christine Jaxel, Sandrine Magnard, Manuel Garrigos, Ste´phane Orlowski, Nade`ge Jamin, Pierre Falson, and Vincent Chaptal 6 Production of Membrane Proteins in Pseudomonas stutzeri. . . . . . . . . . . . . . . . . . . 91 Hao Xie, Cornelia Muenke, Manuel Sommer, Sabine Buschmann, and Hartmut Michel 7 Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 Landon Haslem, Marina Brown, Xin A. Zhang, Jennifer M. Hays, and Franklin A. Hays 8 Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 Landon Haslem, Marina Brown, Xin A. Zhang, Jennifer M. Hays, and Franklin A. Hays 9 Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Jorgaq Pata, Alexis Moreno, Sandrine Magnard, Atanu Banerjee, Rajendra Prasad, and Pierre Falson ix10 Membrane Protein Production in the Yeast P. pastoris . . . . . . . . . . . . . . . . . . . . . . . 187 Hoor Ayub, Michelle Clare, Luke Broadbent, John Simms, Alan D. Goddard, Alice J. Rothnie, and Roslyn M. Bill 11 Production and Preparation of Isotopically Labeled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses . . . . . . . . . . . . . . . . . . . . . 201 Lina Barret, Tobias Schubeis, Vale´rie Kugler, Lucile Guyot, Guido Pintacuda, and Renaud Wagner 12 Membrane Protein Production in Insect Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223 Afroditi Vaitsopoulou, Peer Depping, Roslyn M. Bill, Alan D. Goddard, and Alice J. Rothnie 13 Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241 Pawel Krawinski and Martin Caffrey 14 Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 273 Ana S. Ramı´rez, Kamil Nosol, and Kaspar P. Locher 15 Heterologous Expression and Purification of GPCRs. . . . . . . . . . . . . . . . . . . . . . . . 295 Xiaoting Li, Ling Shen, Junlin Liu, Xiaoyan Liu, Zhi-Jie Liu, and Tian Hua 16 Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313 Lukas Helfinger and Christopher G. Tate 17 Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-like Particles. . . . . . . . . . . . . . . . . . . . . . . . . . . . 327 Kathleen Aertgeerts, Thao T. Ho, and Yingzhou G. Yan 18 Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface. . . . . . . . . . . . . . . . . . . . . . . 337 Valeria Jaramillo-Martinez, Vadivel Ganapathy, and Ina L. Urbatsch 19 Tailoring Purification and Analysis of Membrane Proteins with Modular Detergents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 359 Leonhard H. Urner 20 Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 Valentina S. Dimitrova, Saemee Song, Alexandra Karagiaridi, Anika Marand, and Heather W. Pinkett 21 Detergent-Free Membrane Protein Purification Using SMA Polymer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Luke Broadbent, Peer Depping, Alexis Lode´, Afroditi Vaitsopoulou, David Hardy, Hoor Ayub, James Mitchell-White, Ian D. Kerr, Alan D. Goddard, Roslyn M. Bill, and Alice J. Rothnie 22 Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 405 Simon Umbach, Volker Do ¨tsch, and Frank Bernhard x Contents23 Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425 Hoa Quynh Do and Michaela Jansen 24 Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations . . . . . . . . . . . . . . . 445 Sandra Kovachka, Pierre Soule, and Isabelle Mus-Veteau Index . . . . . . . . . .. . . . . . . . . . . . . 463   مشخصات فایل
عنوان (Title):	Heterologous Expression of Membrane Proteins_ Methods and Protocols
نام فایل (File name):	921-www.GeneProtocols.ir-Heterologous Expression of Membrane Proteins_ Methods and Protocols-Humana Press (2022).pdf
عنوان فارسی (Title in Persian):	بیان هترولوگ پروتئین های غشایی- روشها و پروتکل ها
ایجاد کننده:	Isabelle Mus-Veteau
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071623672, 9781071623671
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	12.7 مگابایت
تعداد صفحات (Book length in pages):	472

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