بیان ژن بدون سلول (سل فری)- روشها و پروتکل ها Cell-Free Gene Expression_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi PART I TOOLS FOR CELL-FREE EXPRESSION SYSTEMS 1 Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Eugenia F. Romantseva, Drew S. Tack, Nina Alperovich, David Ross, and Elizabeth A. Strychalski 2 Simple Extract Preparation Methods for E. coli-Based Cell-Free Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Alissa C. Mullin, Taylor Slouka, and Javin P. Oza 3 Preparation and Screening of Cell-Free Extract from Nongrowing Escherichia coli A19 Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Florian Hiering, Jurek Failmezger, and Martin Siemann-Herzberg 4 Cell-Free Protein Synthesis Using Pichia pastoris . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 Alex J. Spice, Rochelle Aw, and Karen M. Polizzi 5 A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Huiling Xu, Wan-Qiu Liu, and Jian Li 6 In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Hossein Moghimianavval, Yen-Yu Hsu, Alessandro Groaz, and Allen P. Liu 7 High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Conary Meyer, Chuqing Zhou, Zecong Fang, Marjorie L. Longo, Tingrui Pan, and Cheemeng Tan 8 Cell-Free Gene Expression from DNA Brushes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 Michael Levy, Ohad Vonshak, Yiftach Divon, Ferdinand Greiss, Noa Avidan, Shirley S. Daube, and Roy H. Bar-Ziv 9 Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications. . . . . . . . . . . . . . . . 151 Kazuaki Amikura, Keita Hibi, and Yoshihiro Shimizu 10 Measurement of Transcription, Translation, and Other Enzymatic Processes During Cell-Free Expression Using PERSIA . . . . . . . . . . . . . . . . . . . . . . 169 Scott Wick and Peter A. Carr PART II APPLICATIONS OF CELL-FREE EXPRESSION SYSTEMS 11 Cell-Free Noncanonical Redox Cofactor Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 William B. Black and Han Li ix12 Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199 Blake J. Rasor, Bastian Vo ¨geli, Michael C. Jewett, and Ashty S. Karim 13 Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217 April M. Miguez, Yan Zhang, and Mark P. Styczynski 14 Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus. . . . . . . . . 227 Orion M. Venero, Wakana Sato, Joseph M. Heili, Christopher Deich, and Katarzyna P. Adamala 15 Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237 Imre Banlaki, Franc¸ois-Xavier Lehr, and Henrike Niederholtmeyer 16 Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 Miranda L. Jacobs and Neha P. Kamat 17 Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 273 Nicola J. Harris, Eamonn Reading, and Paula J. Booth 18 Assembly of RNA Nanostructures from Double-Crossover Tiles. . . . . . . . . . . . . . 293 Jaimie Marie Stewart, Hari K. K. Subramanian, and Elisa Franco 19 Cell-Free Biosensors and AI Integration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303 Paul Soudier, Le´on Faure, Maish Kushwaha, and Jean-Loup Faulon 20 ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 325 Jaeyoung K. Jung, Khalid K. Alam, and Julius B. Lucks 21 Optical Sensing in Cell-Free Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343 Junzhu Yang and Yuan Lu 22 Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers. . . . . . . 351 Melissa K. Takahashi, Xiao Tan, and Aaron J. Dy 23 Detection of Norovirus Using Paper-Based Cell-Free Systems. . . . . . . . . . . . . . . . 375 Kaiyue Wu and Alexander A. Green 24 A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Franziska Wimmer, Frank Englert, and Chase L. Beisel 25 Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413 Ally Huang, Bruce Bryan, Sebastian Kraves, Ezequiel Alvarez-Saavedra, and Jessica C. Stark Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 433   مشخصات فایل
عنوان (Title):	Cell-Free Gene Expression_ Methods and Protocols
نام فایل (File name):	905-www.GeneProtocols.ir-Cell-Free Gene Expression_ Methods and Protocols-Humana (2022).pdf
عنوان فارسی (Title in Persian):	بیان ژن بدون سلول- روشها و پروتکل ها
ایجاد کننده:	Ashty S. Karim, Michael C. Jewett
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071619977, 9781071619971
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	11.2 مگابایت
تعداد صفحات (Book length in pages):	437

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