ترافیک غشایی- روشها و پروتکل ها Membrane Trafficking_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi PART I THE EXOCYTIC PATHWAY 1 Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Marine D. Camus and Stephane M. Camus 2 Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Julia Dancourt and Gre´gory Lavieu 3 Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH). . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Cedric S. Asensio 4 Spatial and Temporal Control of Protein Secretion with Light. . . . . . . . . . . . . . . . 29 Ashley M. Bourke and Matthew J. Kennedy 5 Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Syed Mukhtar Ahmed 6 Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Kunrong Mei, Di-Ao Liu, and Wei Guo 7 Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy. . . . . . . . . . . . . 79 Fei Kang and Herbert Y. Gaisano 8 Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Jihong Gong, Xiaofei Yang, and Cong Ma 9 Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse–Chase Labeling in Cultured Hippocampal Neurons . . . . . . . . . . . . . 101 Tong Wang and Fre´de´ric A. Meunier 10 Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129 Tamara Basta, Garry P. Morgan, Eileen T. O’Toole, Nalini R. Rao, Jeffrey N. Savas, and Michael H. B. Stowell 11 In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 Yinghui Liu, Ruyue He, Min Zhu, and Haijia Yu vii12 Imaging Single-Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Yingke Xu, Luhong Jin, and Derek Toomre PART II THE ENDOCYTIC PATHWAY 13 Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167 Bijeta Prasai and Justin W. Taraska 14 Endocytosis Assays Using Cleavable Fluorescent Dyes. . . . . . . . . . . . . . . . . . . . . . . 181 Shifeng Wang, Chun Wan, Galen T. Squiers, and Jingshi Shen 15 Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195 Bing Wang, Rui Yang, Yuan Tian, and Qian Yin 16 Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213 Amulya Priya and Sunando Datta 17 Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approaches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237 Priyanka Sharma, Sameena Parveen, and Sunando Datta 18 Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins. . . . . . . . . . . . . . . . . . . . . . . . . . 259 Marijn G. J. Ford and Rajesh Ramachandran 19 Measurement of Lysosome Positioning by Shell Analysis and Line Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285 Chad D. Williamson, Carlos M. Guardia, Raffaella De Pace, Juan S. Bonifacino, and Amra Saric PART III EMERGING TOPICS IN MEMBRANE TRAFFICKING 20 Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 309 Bobae Hyeon, Mai Khanh Nguyen, and Won Do Heo 21 Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 333 Daniel R. Gulbranson 22 Studying Unconventional Secretion of Misfolded Proteins in Cultured Cells and Primary Neurons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 Juhyung Lee and Yihong Ye 23 Single-Molecule Optical Tweezers Study of Protein–Membrane Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 367 Lu Ma, Jinghua Ge, Xin Bian, and Yongli Zhang viii Contents24 Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 385 Max Piffoux, Amanda Karine Andriola Silva, Florence Gazeau, and David Tareste 25 Quantitative Measurement of Extracellular Vesicle Content Delivery Within Acceptor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 E´ meline Bonsergent, She´ryl Bui, and Gre´gory Lavieu Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 405   مشخصات فایل
عنوان (Title):	Membrane Trafficking_ Methods and Protocols
نام فایل (File name):	913-www.GeneProtocols.ir-Membrane Trafficking_ Methods and Protocols-Humana Press (2022).pdf
عنوان فارسی (Title in Persian):	ترافیک غشایی- روشها و پروتکل ها
ایجاد کننده:	Jingshi Shen
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	9781071622087, 9781071622094
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	14.3 مگابایت
تعداد صفحات (Book length in pages):	408

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