تنظیم ژن های پروکاریوتی- روشها و پروتکل ها Prokaryotic Gene Regulation_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . ix 1 Chromosome Conformation Capture in Bacteria and Archaea . . . . 1 Fatema-Zahra M. Rashid, Laurien Detmar, and Remus T. Dame 2 Micrococcal Nuclease Digestion Assays for the Analysis of Chromosome Structure in Archaea. . . . . . . . . . . 29 Hugo Maruyama 3 Detecting DNA Methylations in the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius Using SMRT Sequencing. . . . . . . . 39 Christian Tellgren-Roth and Mohea Couturier 4 ProD: A Tool for Predictive Design of Tailored Promoters in Escherichia coli . . . . . . . . . . . . . 51 Friederike Mey, Jim Clauwaert, Maarten Van Brempt, Michiel Stock, Jo Maertens, Willem Waegeman, and Marjan De Mey 5 Computational Study on the Dynamics of Mycobacterium Tuberculosis RNA Polymerase Assembly. . . . . . . . . . . 61 Sneha Bheemireddy and Narayanaswamy Srinivasan 6 In Vitro Transcription Assay for Archaea Belonging to Sulfolobales. . . . . . . . . . . 81 David Sybers, Daniel Charlier, and Eveline Peeters 7 Prediction of DNA-Binding Transcription Factors in Bacteria and Archaea Genomes . . . . . . . . . 103 Leonardo Ledesma, Rafael Hernandez-Guerrero, and Ernesto Perez-Rueda 8 In Vivo Screening Method for the Identification and Characterization of Prokaryotic, Metabolite-Responsive Transcription Factors . . . . . 113 Amber Joka Bernauw, Veerke De Kock, and Indra Bervoets 9 Application of Special Electron Microscopy Techniques to the Study of DNA – Protein Complexes in E. coli Cells . . . . . . . 143 Andrey Moiseenko, Nataliya Loiko, Olga S. Sokolova, and Yurii F. Krupyanskii 10 High-Speed Atomic Force Microscopy Visualization of ProteinDNA Interactions Using DNA Origami Frames . . . . 157 Ronnie G. Willaert and Sandor Kasas 11 Separation and Characterization of Protein–DNA Complexes by EMSA and In-Gel Footprinting. . . . . . . . . . 169 Daniel Charlier and Indra Bervoets 12 Chemical Protection and Premodification-Binding Interference for the Identification of Phosphate and Base-Specific Contacts in Protein–DNA Complexes . . . . . . . . . 201 Daniel Charlier vii13 Analysis of Protein–DNA Interactions Using Isothermal Titration Calorimetry: Successes and Failures . . . . . . . . . 239 San Hadzˇi and Jurij Lah 14 Determination of RNA Structure with In Vitro SHAPE Experiments . . . . . . . . . . 259 Rani Baes, Daniel Charlier, and Eveline Peeters 15 Microscale Thermophoresis to Study RNA–RNA Binding Affinity . . . . . . . . 291 Britta Jordan, Lisa Nickel, and Ruth A. Schmitz 16 Toeprint Assays for Detecting RNA Structure and Protein–RNA Interactions . . . . . . . 305 Helen Yakhnin and Paul Babitzke Index . . . . . . . . . . 317   مشخصات فایل
عنوان (Title):	Prokaryotic Gene Regulation_ Methods and Protocols
نام فایل (File name):	927-www.GeneProtocols.ir-Prokaryotic Gene Regulation_ Methods and Protocols-Humana (2022).pdf
عنوان فارسی (Title in Persian):	تنظیم ژن های پروکاریوتی- روشها و پروتکل ها
ایجاد کننده:	Eveline Peeters, Indra Bervoets
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071624121, 9781071624128
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	8.98 مگابایت
تعداد صفحات (Book length in pages):	321

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