داینئین - روش ها و پروتکل ها Dynein_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . ix PART I METHODS FOR THE CELL BIOLOGICAL ASSESSMENT OF DYNEIN FUNCTION: FUNGAL SYSTEMS 1 Live-Cell Imaging of Dynein-Mediated Cargo Transport in Aspergillus nidulans. . . . . . . . . . . 3 Rongde Qiu, Jun Zhang, Dennis McDaniel, Miguel A. Pen˜alva, and Xin Xiang 2 Using Budding Yeast as a Model to Understand Dynein-Mediated Cargo Transport. . . . . . . . . . 25 Kari H. Ecklund, Matthew G. Marzo, and Steven M. Markus PART II METHODS FOR THE CELL BIOLOGICAL ASSESSMENT OF DYNEIN FUNCTION: METAZOAN SYSTEMS 3 Employing Live-Cell Imaging to Study Motor-Mediated Transport . . . . . . 45 Sydney E. Cason, Adam R. Fenton, and Erika L. F. Holzbaur 4 Assessment of Dynein-Mediated Nuclear Migration in the Developing Cortex by Live-Tissue Microscopy . . . . . . . . . . 61 Haw-Yuan Cheng, Fang-Shin Nian, Yi-Wen Ou, and Jin-Wu Tsai 5 Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cells. . . . . . . . . . 73 Tomomi Kiyomitsu 6 Visualization of Single Dynein Molecules in Mammalian Cells. . . . . . 87 Nireekshit Addanki Tirumala and Vaishnavi Ananthanarayanan PART III AT THE CROSSROADS: COMBINED IN-CELL AND IN VITRO METHODS TO MEASURE DYNEIN FUNCTION 7 Single-Molecule Studies of Motor Adaptors Using Cell Lysates. . . . . 97 Adam R. Fenton, Sydney E. Cason, and Erika L. F. Holzbaur 8 Reconstitution of Organelle Transport Along Microtubules In Vitro . . . . . 113 Daniel Beaudet and Adam G. Hendricks viiviii Contents PART IV METHODS FOR THE BIOCHEMICAL RECONSTITUTION OF ACTIVE DYNEIN ASSEMBLIES 9 Reconstitution of Dynein/Dynactin Transport Using Recombinant Dynein. . . . . . . . . . . . 135 Clinton K. Lau 10 Fast and Easy Transient Mammalian Cell Expression and Purification of Cytoplasmic Dynein . . . . . . . . . . 157 Akane Furuta and Ken’ya Furuta PART V METHODS TO MEASURE MOTILITY AND FORCE GENERATION OF DYNEIN ASSEMBLIES 11 High-Resolution Tracking of Dynein-Dynactin-BicD2 Complexes. . 177 Qingzhou Feng, Allison M. Gicking, and William O. Hancock 12 In Vivo Trapping of Latex Bead Phagosomes for Quantitative Force Measurements . . . . . . . . . . . . 187 Paulomi Sanghavi, Arpan Rai, and Roop Mallik 13 Studying Dynein Mechanochemistry with an Optical Trap . . . . 201 Emre Kusakci and Ahmet Yildiz 14 Measurements of the Force-Dependent Detachment Rates of Cytoplasmic Dynein from Microtubules. . . . . . . . 221 Xinglei Liu, Lu Rao, and Arne Gennerich PART VI METHODS FOR THE STRUCTURAL ANALYSIS OF DYNEINS 15 NMR Analysis of the Interactions and Conformational Plasticity of Dynein Intermediate Chain. . . . . . . . . 241 Kayla A. Jara and Elisar J. Barbar 16 High-Resolution Structural Analysis of Dyneins by Cryo-electron Microscopy. . . . . . . . . . 257 Pengxin Chai, Qinhui Rao, Yue Wang, and Kai Zhang Index . . . . . . . . . . . . . . . 281   مشخصات فایل
عنوان (Title):	Dynein_ Methods and Protocols
نام فایل (File name):	1004-www.GeneProtocols.ir-Dynein_ Methods and Protocols-Humana Press (2023).pdf
عنوان فارسی (Title in Persian):	داینئین - روش ها و پروتکل ها
ایجاد کننده:	Steven M. Markus
زبان (Language):	انگلیسی English
سال انتشار:	2023
شابک ISBN:	9781071629574
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	14.6 مگابایت
تعداد صفحات (Book length in pages):	279

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