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قابل دانلود از يكشنبه, ۱۱ تیر ۱۴۰۲
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فهرست مطالب: Preface........... . . . vContributors........... . . . . xi PART I SALIVA AND OTHER ORAL FLUIDS 1 RNA Sequencing Analysis of Saliva exRNA...... . . 3 2 Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry........ 13 3 Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics. . . . . . 25 4 Antioxidant Micronutrients and Oxidative Stress Biomarkers.. . . . . . 41 PART II MOLECULAR BIOSCIENCES 5 The Oral Microbiota in Health and Disease: An Overview of Molecular Findings........ . . . 61 6 The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes.... . . . . . 75 7 Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)....... . . . 91 8 Bioinformatic Approaches for Describing the Oral Microbiota... . . . 105 9 Adhesion of Yeast and Bacteria to Oral Surfaces.... . . . . 131 10 Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)...... . . . . 157 11 Methods to Study Antagonistic Activities Among Oral Bacteria... . . 171 12 Generation of Multispecies Oral Bacteria Biofilm Models... . . 187 13 Markerless Genome Editing in Competent Streptococci... . . . 201 14 A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System....... . . . . . 217 15 Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients...... . . . 231 16 Strategy for RNA-Seq Experimental Design and Data Analysis.. . . . 249 17 Characterization of the Expression and Role of Histone Acetylation and Deacetylation in Dental Pulp Cells...... 279 18 Genome-Wide Analysis of Periodontal and Peri-implant Cells and Tissues.......... . 295 19 Differential Expression, Functional and Machine Learning Analysis of High-Throughput –Omics Data Using Open-Source Tools... . . . 317 20 Micro-RNA Profiling in Dental Pulp Cell Cultures..... . 353 PART III CELLS AND TISSUES 21 Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease...... . . . . . 371 22 A Cell Culture Method for the Isolation and Study of Primary Human Dental Pulp Cells......... . 393 23 Culturing Adipose-Derived Stem Cells Under Serum-Free Conditions. . . 407 24 Quantitative Real-Time Gene Profiling of Human Alveolar Osteoblasts Using a One-Step System........ 417 25 Fabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructs........ . . . 429 26 Immunohistochemistry and Immunofluorescence..... . . 439 27 Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps......... 451 28 Cell Seeding on 3D Scaffolds for Tissue Engineering and Disease Modeling Applications........ . . 473 29 Workflow for Fabricating 3D-Printed Resorbable Personalized Porous Scaffolds for Orofacial Bone Regeneration..... . . . 485 30 Methacrylated Gelatin as an On-Demand Injectable Vehicle for Drug Delivery in Dentistry....... . 493 31 In Vitro Biological Testing of Dental Materials.... . . . . . 505 Index........... . . . . 525 فهرست به فارسی (ترجمه ماشینی): پیشگفتار........... . . . v مشخصات فایل |
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| عنوان (Title): | Oral Biology_ Molecular Techniques and Applications |
| نام فایل (File name): | 1063-www.GeneProtocols.ir-Oral Biology_ Molecular Techniques and Applications-Humana Press (.pdf |
| عنوان فارسی (Title in Persian): |
زیست شناسی دهان - تکنیک ها و کاربرد های مولکولی
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| ایجاد کننده: | Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng, Paul R. Cooper |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: |
ISBN 978-1-0716-2779-2 ISBN 978-1-0716-2780-8 (eBook) |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 18.7 مگابایت |
| تعداد صفحات (Book length in pages): | 522 |
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