سلول های بنیادی پرتوان القا شده و بیماری های انسانی - روشها و پروتکل ها Induced Pluripotent Stem Cells and Human Disease_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . v Contributors. . . . . . . . . xi Analysis of Mitochondrial Dysfunction by Microplate Reader in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Disorders . . . . . . 1 Tatiana R. Rosenstock, Congxin Sun, Georgina Wynne Hughes, Katherine Winter, and Sovan Sarkar Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases . . . . . 23 Gu¨len Gu¨ney-Esken and Fatima Aerts-Kaya Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis Patients . . . . . . . . 43 Onur Can Begentas, Dilara Koc, and Erkan Kiris Monitoring Axonal Degeneration in Human Pluripotent Stem Cell Models of Hereditary Spastic Paraplegias . . . . . . 69 Xue-Jun Li, Yongchao Mou, Conrad Milton, and Zhenyu Chen Efficient Generation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells for Disease Modeling and Disease Gene Discovery . . . . 85 Satish Kumar, Joanne E. Curran, Sarah Williams-Blangero, and John Blangero Autophagy Dysfunction as a Phenotypic Readout in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Diseases . . . . 103 Congxin Sun, Tatiana R. Rosenstock, Malkiel A. Cohen, and Sovan Sarkar Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors . . . . 137 Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Harshini Surendran, and Rajarshi Pal Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells . . . . . . . . . 153 Shennea S. McGarvey, Michael Ferreyros, Igor Kogut, and Ganna Bilousova A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells . . . . . . . 169 Maryna Pavlova, Shennea S. McGarvey, Ganna Bilousova, and Igor Kogut Image-Based Quantitation of Kainic Acid-Induced Excitotoxicity as a Model of Neurodegeneration in Human iPSC-Derived Neurons . . . . 187 Jana Talbot, Sueanne Chear, Andrew Phipps, Alice Pe´bay, Alex W. Hewitt, James C. Vickers, Anna E. King, and Anthony L. Cook Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures. . . 209 Hirotaka Watanabe, Rei Murakami, and Hideyuki Okano viiCreating Cell Model 2.0 Using Patient Samples Carrying a Pathogenic Mitochondrial DNA Mutation: iPSC Approach for LHON. . . . . . . 219 Pragya Singh, Tyler Bahr, Xiaoxu Zhao, Peiqing Hu, Marcel Daadi, TaoSheng Huang, and Yidong Bai CRISPR Guide RNA Library Screens in Human Induced Pluripotent Stem Cells . . . . . . . 233 Yan Zhou, Qiang Fu, Huijun Shi, and Guangqian Zhou Generation and Encapsulation of Human iPSC-Derived Vascular Smooth Muscle Cells for Proangiogenic Therapy . . . . . . . 259 Biraja C. Dash and Henry C. Hsia Methods to Induce Small-Scale Differentiation of iPS Cells into Dopaminergic Neurons and to Detect Disease Phenotypes . . . . 271 Akihiro Yamaguchi, Kei-ichi Ishikawa, and Wado Akamatsu Modeling Early Neural Crest Development via Induction from hiPSC-Derived Neural Plate Border-like Cells. . . . . . . . . 281 Diogo Andrade Nani, Gabriella Shih Ping Hsia, Maria Rita Passos-Bueno, and Gerson Shigeru Kobayashi Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells . . . . . . . 299 Miao Yu, Xinglong Han, Lingqun Ye, Wei Lei, and Shijun Hu A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium . . . . . . 307 Miho Tagawa, Hanako Ohashi Ikeda, Masayuki Hata, Yumi Inoue, Sachiko Iwai, and Akitaka Tsujikawa Genome Editing of Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes to Model Genetic Ocular Diseases . 321 Heran Getachew, Blanca Chinchilla, and Rosario Fernandez-Godino Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling . . . . . 335 Arline Joachim, Shiqiao Ye, and Ming-Tao Zhao Immunoassay for Quantitative Detection of Antibody Transcytosis Across the Blood-Brain Barrier In Vitro . . . . . . 345 Caroline Sodja, Deborah Callaghan, Arsalan S. Haqqani, Danica B. Stanimirovic, Willard J. Costain, and Anna Jezierski Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells . . . . . . . 359 Shermaine Huiping Tay, Winanto, Zi Jian Khong, Yong Hui Koh, and Shi Yan Ng CRISPR/Cas-Mediated Knock-in of Genetically Encoded Fluorescent Biosensors into the AAVS1 Locus of Human-Induced Pluripotent Stem Cells. . . . . . . . . . 379 David Stellon, Minh Thuan Nguyen Tran, Jana Talbot, Sueanne Chear, Mohd Khairul Nizam Mohd Khalid, Alice Pe´bay, James C. Vickers, Anna E. King, Alex W. Hewitt, and Anthony L. Cook viii ContentsCancer Stem Cell Initiation by Tumor-Derived Extracellular Vesicles . . 399 Said M. Afify, Ghmkin Hassan, Ting Yan, Akimasa Seno, and Masaharu Seno Genome Editing Using Cas9-gRNA Ribonucleoprotein in Human Pluripotent Stem Cells for Disease Modeling . . . . . . 409 Noelia Beneto, Daniel Grinberg, Lluı ¨sa Vilageliu, and Isaac Canals Index . . . . . . . . . . 427   مشخصات فایل
عنوان (Title):	Induced Pluripotent Stem Cells and Human Disease_ Methods and Protocols
نام فایل (File name):	947-www.GeneProtocols.ir-Induced Pluripotent Stem Cells and Human Disease_ Methods and Protocols-Humana Press (2022).pdf
عنوان فارسی (Title in Persian):	سلول های بنیادی پرتوان القا شده و بیماری های انسانی - روشها و پروتکل ها
ایجاد کننده:	Kursad Turksen
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071625845, 9781071625842
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	14.6 مگابایت
تعداد صفحات (Book length in pages):	426

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