سلول های کرومافین - روشها و پروتکل ها Chromaffin Cells_ Methods and Protocols

فهرست مطالب: Preface .......... . . . . . v Contributors.......... . xi PART I CULTURING AND MORPHOLOGY 1 Rat Pheochromocytoma PC12 Cells in Culture..... 3 Pin-Chun Chen and Chih-Tien Wang 2 Adrenal Grafts in the Central Nervous System: Chromaffin and Chromaffin Progenitor Cell Transplantation....17 Alejandra Boronat-Garcia, Marcela Palomero-Rivero, and Magdalena Guerra-Crespo 3 Immunocytochemistry of Acutely Isolated Adrenal Medullary Chromaffin Cells........35 Keita Harada, Hidetada Matsuoka, and Masumi Inoue 4 Transmission Electron Microscopy: A Method for Studying the Adrenal Chromaffin Cells....... . . 43 Anna Capaldo, Maria De Falco, Luigi Rosati, and Vincenza Laforgia 5 Immunogold for Protein Location in Chromaffin Cells .... 57 Rafael Luja´n, Rocı´o Alfaro-Ruiz, and Carolina Aguado 6 Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopy..... . . . . . 77 Chung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo, and Ling-Gang Wu PART II STIMULUS-SECRETION COUPLING 7 Online Detection of Catecholamine Release from the Perfused Rat Adrenal Gland ........ . . . . 91 Ricardo Borges, Ana De la Iglesia, and Antonio G. Garcı´a 8 Real Time Recording of Perifused Chromaffin Cells.... . . . 105 Ricardo de Pascual, Alicia Mun˜oz-Montero, and Luis Gandı´a 9 Recording of Chromaffin Cell Electrical Activity In Situ in Acute Adrenal Slices ........ 113 Nathalie C. Gue´rineau 10 Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normotensive, Wistar Kyoto Rats and Spontaneously Hypertensive Rats ... . . . . . 129 Tzitzitlini Alejandre-Garcı´a, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Milla´n-Aldaco, and Arturo Herna´ndez-Cruz vii viii Contents 11 Measurements of Calcium in Chromaffin Cell Organelles Using Targeted Aequorins ....... . . . . 153 Jaime Santo-Domingo, Pilar A´ lvarez-Illera, Pablo Montenegro, Rosalba I. Fonteriz, Mayte Montero, and Javier A´ lvarez 12 Quantification of Secretory Granule Exocytosis by TIRF Imaging and Capacitance Measurements....... 179 Muhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg 13 Membrane Capacitance Measurements of Stimulus-Evoked Exocytosis in Adrenal Chromaffin Cells...... 187 Elizabeth P. Seward and Robert C. Wykes 14 Quantal Release Analysis of Electrochemically Active Molecules Using Single-Cell Amperometry ......203 Jose´ David Machado, Pablo Montenegro, and Natalia Domı´nguez 15 Methodologies for Detecting Quantal Exocytosis in Adrenal Chromaffin Cells Through Diamond-Based MEAs ... . 213 Giulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, and Valentina Carabelli 16 Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released ...... . . . . . 223 Soodabeh Majdi, Alex S. Lima, and Andrew G. Ewing 17 Patch Amperometry and Intracellular Patch Electrochemistry ... . 239 Eugene V. Mosharov and Manfred Lindau 18 Artificial Cells for Dissecting Exocytosis .....261 Yuanmo Wang and Ann-Sofie Cans PART III CHROMAFFIN GRANULES AND MEMBRANE TRAFFIC 19 Isolation and Purification of Chromaffin Granules from Adrenal Glands and Cultured Neuroendocrine Cells ..... . . . 283 Arlek Gonza´ lez-Jamett, Marı´a Constanza Maldifassi, and Ana Marı´a Ca´ rdenas 20 Confocal Microscopy Studies of F-Actin Cytoskeleton Distribution and Dynamics Using Fluorescent LifeAct Constructs in Bovine Adrenal Chromaffin Cells ....... . . . . . 297 Jose´ Villanueva, Yolanda Gime´nez-Molina, and Luis M. Gutie´rrez 21 Unveiling the Nanoscale Dynamics of the Exocytic Machinery in Chromaffin Cells with Single-Molecule Imaging .... . . . 311 Adekunle T. Bademosi and Fre´de´ric A. Meunier Contents ix PART IV MISCELLANEA 22 Determination of Catecholamines in a Small Volume (25 μL) of Plasma from Conscious Mouse Tail Vein ..... . . . 331 Kechun Tang and Sushil K. Mahata 23 Quantification of Chromogranin A and Its Fragments in Biological Fluids........ . . . . 343 Flavio Curnis, Barbara Colombo, and Angelo Corti 24 Cytotoxicity Models in Chromaffin Cells to Evaluate Neuroprotective Compounds ....... . 361 Marı´a F. Cano-Abad and Manuela G. Lopez Index ..........371     مشخصات فایل
عنوان (Title):	Chromaffin Cells_ Methods and Protocols
نام فایل (File name):	965-www.GeneProtocols.ir-Chromaffin Cells_ Methods and Protocols-Humana Press (2023).pdf
عنوان فارسی (Title in Persian):	سلول های کرومافین - روشها و پروتکل ها
ایجاد کننده:	Ricardo Borges (editor)
زبان (Language):	انگلیسی English
سال انتشار:	2023
شابک ISBN:	9781071626702
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	12.8 مگابایت
تعداد صفحات (Book length in pages):	367

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