سلول های T تنظیمی - روشها و پروتکل ها Regulatory T-Cells_ Methods and Protocols

فهرست مطالب: Preface ............ . . v Contributors........... . . . . ix PART I IMMUNOLOGICAL TECHNIQUES FOR ANALYSING TREG 1 Analysis of the In Vivo Function of Follicular Regulatory T (TFR) Cells in the Regulation of Antibody Response .....3 Lin Luo and Jianmei W. Leavenworth 2 Adoptive Transfer and Bone Marrow Chimera Models to Analyze Treg Function and Differentiation ....... . . . 15 Vital da Silva Domingues, Iris Caramalho, Marie-Louise Bergman, and Jocelyne Demengeot 3 Depletion of Treg by the Diphtheria Toxin System..... . 31 Sho Hanakawa, Zachary Chow, and Kenji Kabashima 4 Analysis of Peripherally Derived Treg in the Intestine....41 Tadashi Takeuchi and Hiroshi Ohno 5 Isolation and Analysis of Tumor-Infiltrating Treg ..... . . 51 Katharina Bergerhoff and Malin Pedersen PART II MOLECULAR TECHNIQUES FOR ANALYSING TREG 6 Differentiation of Peripheral Treg ....... . . . . 67 Jose´ Almeida-Santos, Marie-Louise Bergman, and Jocelyne Demengeot 7 Functional Analysis of Foxp3 and Its Mutants by Retroviral Transduction of Murine Primary CD4+ T Cells ..... . . . . 79 Akira Nakajima, Ryuichi Murakami, and Shohei Hori 8 Genetic Tools for Analyzing Foxp3+ Treg Cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping .... . . . . 95 Mario Simonetti, Acelya Yilmazer, and Karsten Kretschmer 9 Analysis of FOXP3 DNA Methylation Patterns to Identify Functional FOXP3+ T-Cell Subpopulations ...... . 115 Ekaterina Minskaia and Joa˜o F. Lacerda 10 Protein Ubiquitination Assay........ . . 137 Ping Wei, Rui Liang, and Fan Pan PART III HUMAN TREG ANALYSIS AND CLINICAL APPLICATION 11 Phenotypic and Functional Studies of Human Treg Cell Subpopulations .153 Mo Atif, Mustapha Cherai, and Makoto Miyara 12 In Vitro Analysis of CTLA-4-Mediated Transendocytosis by Regulatory T Cells ......... . . 171 Erin Waters, Cayman Williams, Alan Kennedy, and David M. Sansom vii viii Contents 13 Isolation and Functional Characterization of Regulatory CD4+ T Cells from the Inflamed Joints of Patients with Rheumatoid Arthritis ........ . . . . 189 Giovanni A. M. Povoleri, Veerle Fleskens, and Leonie S. Taams 14 A GMP Protocol for the Manufacture of Tregs for Clinical Application.. . 205 Alice O’Neil, Matthew Brook, Seetha Abdul-Wahab, Joanna Hester, Giovanna Lombardi, and Fadi Issa PART IV SINGLE CELL AND HIGH-DIMENSIONAL TECHNIQUES FOR ANALYSING TREG 15 Regulatory T-Cell Phenotyping Using CyTOF ..... . . . . 231 Hugo Barcenilla, Mikael Pihl, Florence Sjo ¨gren, Louise Magnusson, and Rosaura Casas 16 High-Dimensional Single-Cell Profiling of Tumor-Infiltrating CD4+ Regulatory T Cells ........243 Giorgia Alvisi, Simone Puccio, Rahul Roychoudhuri, Caterina Scirgolea, and Enrico Lugli 17 Single-Cell Transcriptome Analysis of Treg...... . . 259 Benjy Jek Yang Tan, Masahiro Ono, and Yorifumi Satou Index ............ . . . 279     مشخصات فایل
عنوان (Title):	Regulatory T-Cells_ Methods and Protocols
نام فایل (File name):	960-www.GeneProtocols.ir-Regulatory T-Cells_ Methods and Protocols-Humana Press (2022).pdf
عنوان فارسی (Title in Persian):	سلول های T تنظیمی - روشها و پروتکل ها
ایجاد کننده:	Masahiro Ono
زبان (Language):	انگلیسی English
سال انتشار:	2023
شابک ISBN:	1071626469, 9781071626467
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	11.8 مگابایت
تعداد صفحات (Book length in pages):	276

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