فناوری پروتوپلاست- روشها و پروتکل ها Protoplast Technology _ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . ix PART I BASIC TECHNIQUES AND THEIR APPLICATIONS 1 Physcomitrium patens Protoplasting and Protoplast Transfection . . . . . . . . . . . . . . 3 2 Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici . . . . . . . . . 21 3 Protoplast for Gene Functional Analysis in Arabidopsis . . . . . . . . . . . . . . . . . . . . . . 29 4 Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum . . . . . . . . 49 5 CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration . . . . . . . . . . . . . . . . . . 65 6 Protoplast Isolation and Transfection in Rice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 7 Protoplast Isolation and Transfection in Maize . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 8 Isolation and Transfection of Maize Endosperm Protoplasts. . . . . . . . . . . . . . . . . . 105 9 Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue . . . . . . . . . . 123 10 Protoplast Isolation and Transfection in Wheat . . . . . . . . . . 131 11 Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses. . . . . . . . . . 143 12 Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa). . . . . . . . . . 153 13 Protoplast Isolation, Transfection, and Gene Editing for Soybean (Glycine max) . . . . . 173 14 Protoplast Isolation and Transformation in Oil Palm . . . . . . . . . . . . . . . . . . . . . . . . 187 PART II ADVANCED SYSTEMS FOR HIGH THROUGHPUT ANALYSIS AND AUTOMATION 15 Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells . . . . . . . . . . . . . . . . . . . 205 16 Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast Transfection . . . . . . . . . . . . 223 17 High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts . . . . . . . 245 Index . . . . . . . . . . . . . . . 261   مشخصات فایل
عنوان (Title):	Protoplast Technology _ Methods and Protocols
نام فایل (File name):	899-www.GeneProtocols.ir-Protoplast Technology _ Methods and Protocols-Humana Press (2022).pdf
عنوان فارسی (Title in Persian):	فناوری پروتوپلاست- روشها و پروتکل ها
ایجاد کننده:	Kan Wang, Feng Zhang
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	9781071621639, 9781071621646
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	7.54 مگابایت
تعداد صفحات (Book length in pages):	267

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