مخازن اچ آی وی- روشها و پروتکل ها HIV Reservoirs_ Methods and Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . ix PART I CELL LINES WITH MINIMAL BUT INDUCIBLE PROVIRAL EXPRESSION 1 Jurkat-Derived (J-Lat, J1.1, and Jurkat E4) and CEM-Derived T Cell Lines (8E5 and ACH-2) as Models of Reversible Proviral Latency. . . . . . . . . . . . . . . . . . 3 2 U1 and OM10.1. Myeloid Cell Lines as Surrogate Models of Reversible Proviral Latency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 PART II IN VITRO AND EX VIVO PRIMARY CELL MODELS OF MINIMAL BUT INDUCIBLE PROVIRAL EXPRESSION 3 An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency in Primary Human CD4+ T Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 4 The Cultured TCM Model of HIV Latency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 5 A Reliable Primary Cell Model for HIV Latency: The QUECEL (Quiescent Effector Cell Latency) Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 6 TGF-β Signaling Supports HIV Latency in a Memory CD4+ T Cell Based In Vitro Model . . . . . . . . . . . . . . . . . . 69 7 Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoir Quantification . . . . . . . . . . . . . . . . . . . . 81 8 Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF) . . . . . . . . . . . . . . . . . . . . . 91 9 Human Monocyte-Derived Macrophages (MDM): Model 2 . . . . . .. . . . . . . . . . . . . 97 10 Modeling HIV Latency in Astrocytes with the Human Neural Progenitor Cell Line HNSC.100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103 11 Hematopoietic Stem and Progenitor Cells (HSPCs) . . . . . . . . . . . . . . . . . . . . . . . . 115 PART III IN VITRO AND EX VIVO TISSUE-DERIVED MODELS OF REVERSIBLE PROVIRAL LATENCY 12 Ex Vivo HIV Infection Model of Cervico-Vaginal and Rectal Tissue . . . . . . . . . . 157 13 More than a Gender Issue: Testis as a Distinctive HIV Reservoir and Its Implication for Viral Eradication. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 PART IV ANIMAL MODELS 14 Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells . . . . . . . . 189 15 Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond . . . . . . 205 16 HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure . . . . . . . . . . . 215 17 Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research . . . . . . . . 229 18 Inducing Long-Term HIV-1 Latency in the TKO-BLT Mouse Model . . . . . . 253 PART V METHODS FOR DETECTION AND ANALYSIS OF THE RESERVOIR 19 In Situ Multiplexing to Identify, Quantify, and Phenotype the HIV-1/SIV Reservoir Within Lymphoid Tissue. . . . . . . . . . . . . . . . . . . . . . . . . 277 20 Single-Cell Multiparametric Analysis of Rare HIV-Infected Cells Identified by Duplexed RNAflow-FISH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 291 21 Ex Vivo Differentiation of Resting CD4+ T Lymphocytes Enhances Detection of Replication Competent HIV-1 in Viral Outgrowth Assays . . . . . . . . . . . . . . . . . 315 22 Quantitative Viral Outgrowth Assay to Measure the Functional SIV Reservoir in Myeloid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 333 23 Near-Full-Length Single-Genome HIV-1 DNA Sequencing . . . . . . . . . . . . . . . . . 357 24 TILDA: Tat/Rev Induced Limiting Dilution Assay . . . . . . . . . . . . . . . . . . . . . . . . . 365 PART VI CURE AND LONG-TERM REMISSION STRATEGIES 25 Latency Reversal and Clearance of Persistent HIV Infection. . . . . . . . . . . . . . . . . . 375 26 Cure and Long-Term Remission Strategies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 27 Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils of CRISPR Therapy. . . . . . . . . . . 429 Index . . . . . . . . . . . . . . 447   مشخصات فایل
عنوان (Title):	HIV Reservoirs_ Methods and Protocols
نام فایل (File name):	902-www.GeneProtocols.ir-HIV Reservoirs_ Methods and Protocols-Humana (2022).pdf
عنوان فارسی (Title in Persian):	مخازن اچ آی وی- روشها و پروتکل ها
ایجاد کننده:	Guido Poli, Elisa Vicenzi, Fabio Romerio
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071618709, 9781071618707
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	10.4 مگابایت
تعداد صفحات (Book length in pages):	446

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