مهندسی متابولیک مخمر- روشها و پروتکل ها Yeast Metabolic Engineering_ Methods and Protocols

فهرست مطالب: Preface........... . . . v Contributors........... . . . . ix 1 An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae........ . . . . . 1 Verena Siewers 2 High-Efficiency Plasmid DNA Transformation in Yeast... . . . . 15 O’Taveon R. Fitzgerald, Nestor D. Rodriguez, and L. Kevin Lewis 3 CasPER: A CRISPR/Cas9-Based Method for Directed Evolution in Genomic Loci in Saccharomyces cerevisiae..... . . 23 Tadas Jakocˇiu¯nas, Michael K. Jensen, and Jay D. Keasling 4 Fluorescence-Activated Cell Sorting as a Tool for Recombinant Strain Screening......... . . 39 Christos Skrekas, Raphael Ferreira, and Florian David 5 Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology. . . . . . 59 Kouichi Kuroda and Mitsuyoshi Ueda 6 Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris....... . . . 79 Claudia Rinnofner, Michael Felber, and Harald Pichler 7 Competent Cell Preparation and Transformation of Pichia pastoris.. . . . . . 113 Joan Lin-Cereghino, Christopher A. Naranjo, and Geoff P. Lin-Cereghino 8 CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome.. . . . . . 121 Jasmin Elgin Fischer and Anton Glieder 9 Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris....... . . . . . 135 Simon Kobalter, Astrid Radkohl, Helmut Schwab, Anita Emmerstorfer-Augustin, and Harald Pichler 10 Engineering of Promoters for Gene Expression in Pichia pastoris.. . 153 Thomas Vogl 11 Molecular Tools for Leveraging the Potential of the Acid-Tolerant Yeast Zygosaccharomyces bailii as Cell Factory...... 179 Paola Branduardi, Liliane Barroso, Laura Dato, Edward J. Louis, and Danilo Porro 12 Golden Gate Multigene Assembly Method for Yarrowia lipolytica.. 205 Macarena Larroude, Jean-Marc Nicaud, and Tristan Rossignol 13 A Universal Gene Expression System for Novel Yeast Species... . . . . 221 Dominik Mojzita, Anssi Rantasalo, and Marja Ilme´n vii14 Dynamic Feeding for Pichia pastoris....... . . 243 Alexander Pekarsky and Oliver Spadiut 15 Automated Evolutionary Engineering of Yeasts..... . . . . 255 Erik de Hulster, Christiaan Mooiman, Rowin Timmermans, and Robert Mans 16 Reconstruction of Genome-Scale Metabolic Model for Hansenula polymorpha Using RAVEN....... . . . . . 271 Francisco Zorrilla and Eduard J. Kerkhoven Index........... . . . . 291   مشخصات فایل
عنوان (Title):	Yeast Metabolic Engineering_ Methods and Protocols
نام فایل (File name):	925-www.GeneProtocols.ir-Yeast Metabolic Engineering_ Methods and Protocols-Humana (2022).pdf
عنوان فارسی (Title in Persian):	مهندسی متابولیک مخمر- روشها و پروتکل ها
ایجاد کننده:	Valeria Mapelli
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	9781071623992, 9781071623985
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	7.56 مگابایت
تعداد صفحات (Book length in pages):	300

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