مکانوبیولوژی - روشها و پروتکل ها Mechanobiology_ Methods and Protocols

فهرست مطالب: Preface .............. . . . v Contributors.............ix PART I QUANTIFYING THE MECHANICAL PROPERTIES OF CELLS AND ADHESION PROTEINS 1 Measuring Cell Mechanical Properties Using Microindentation...3 Julien Husson 2 In Situ Measurements of Cell Mechanical Properties Using Force Spectroscopy........... . . . 25 Meital Reches and Tal Duanis-Assaf 3 Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy........... . . . 45 Martin Bergert and Alba Diz-Mun˜oz 4 Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single-Molecule Atomic Force Microscopy .... . 63 Andrew Vae Priest and Sanjeevi Sivasankar PART II EXPOSING CELLS TO EXTERNAL MECHANICAL FORCES 5 Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber........... . . 81 Jason Sedlak and Alisa Clyne 6 Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching .. . 91 Robin Pu¨llen, Jens Konrad, Bernd Hoffmann, and Rudolf Merkel 7 Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo ........ . 107 Kenji Nishizawa, Claire Charde`s, Raphae ¨l Cle´ment, and Pierre-Franc¸ois Lenne PART III QUANTITATIVE CHARACTERIZATION OF CELL MECHANOSENSING 8 A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity Sensing by Plant’s Roots and Root Hair Cells ........... . . 121 David Pereira, Thomas Alline, Gaurav Singh, Marie-Edith Chaboute´, and Atef Asnacios 9 Photoresponsive Hydrogels for Studying Mechanotransduction of Cells .. . 133 Keun-Young Park, David J. Odde, and Mark D. Distefano 10 Analysing Mechanically Evoked Currents at Cell-Substrate Junctions..155 Surabhi Shrestha, Jessica Richardson, and Kate Poole viiviii Contents 11 Quantifying Strain-Sensing Protein Recruitment During Stress Fiber Repair ........... . . . 169 Shailaja Seetharaman, Stefano Sala, Margaret L. Gardel, and Patrick W. Oakes PART IV MEASURING FORCES APPLIED BY CELLS ON THE EXTRACELLULAR MATRIX 12 Quantification of Invadopodia Formation and Matrix Degradation Activity . . . . 185 Jubina Balan Venghateri and Benjamin Geiger 13 Measuring Cellular Traction Forces with Micropillar Arrays .... . . 197 Malak Amer and Haguy Wolfenson 14 Imaging Cell Adhesive Force at the Single Molecule Level .... . . . 207 Ying Tu and Xuefeng Wang PART V FORCE MEASUREMENT INSIDE CELLS 15 Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM .. 221 Lukas Windgasse and Carsten Grashoff 16 Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors .......... . . . 239 Neus Sanfeliu-Cerda´n, Li-Chun Lin, Alexander R. Dunn, Miriam B. Goodman, and Michael Krieg 17 Single-Cell Quantification of the Mechanical Stability of Cell–Cell Adherens Junction Using Glass Micropipettes ......267 Wenmao Huang, Chaoyu Fu, and Jie Yan PART VI PROBING CELL SIGNALING AND GENE EXPRESSION IN RESPONSE TO FORCE 18 Using Micropatterned Supported Lipid Bilayers to Probe the Mechanosensitivity of Signaling Receptors ....... . . 283 Zhongwen Chen 19 Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing . . . . 291 Yekaterina A. Miroshnikova 20 Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK Phosphorylation.... 297 Yuan Yao and Evelyn K. F. Yim PART VII BIOPHYSICAL MODELING OF CELL SHAPE AND PROTEIN DYNAMICS 21 Long-Term Fluorescence Recovery After Photobleaching (FRAP) ... . 311 Takumi Saito, Daiki Matsunaga, and Shinji Deguchi 22 Simulating 3D Cell Shape with the Cellular Potts Model ....323 Rabea Link and Ulrich S. Schwarz Index ..............341    مشخصات فایل
عنوان (Title):	Mechanobiology_ Methods and Protocols
نام فایل (File name):	991-www.GeneProtocols.ir-Mechanobiology_ Methods and Protocols-Humana Press (2023).pdf
عنوان فارسی (Title in Persian):	مکانوبیولوژی - روشها و پروتکل ها
ایجاد کننده:	Ronen Zaidel-Bar
زبان (Language):	انگلیسی English
سال انتشار:	2023
شابک ISBN:	9781071628508
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	15.2 مگابایت
تعداد صفحات (Book length in pages):	337

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