پروتکل های سلول بنیادی جنینی Embryonic Stem Cell Protocols

فهرست مطالب: Preface . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . ix Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens . . . . . . . . . . . . 1 Oriana Genolet, Liat Ravid Lustig, and Edda G. Schulz Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions. . . . . . . . . . 25 Taha Bartu Hayal and Ays¸egu¨l Dog˘an Rat-Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish. . . . . . . . 37 Fatima Bianca A. Dessouki, Pawan K. Singal, and Dinender K. Singla Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells. . . . . . . . . 53 Federico Pecori and Shoko Nishihara High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model . . . 59 Olga Gordeeva Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications . . . . . . 81 Loriana Vitillo and Ludovic Vallier Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol . . . . . . . . . . . . . 101 Hatice Burcu S¸is¸li, Selinay S¸enkal, Derya Sag˘rac¸, Taha Bartu Hayal, and Ays¸egu¨l Dog˘an ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cells . . . . . . . 117 Divya Desai and Prasad Pethe Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids . . . . . . . . . . . . . . 135 Yoshitomo Ueda, Stephen T. Moore, and Eri Hashino Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs). . . . . . . . 151 Bibiana Correia, Maria Ineˆs Sousa, and Joa˜o Ramalho-Santos Replating Protocol for Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes . . . . . . . . . . . . . 161 Arzuhan Koc and Esra Cagavi Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo . . . . . . . . . . . 171 Katsuhiro Konno, Jingjing Kobayashi-Sun, Fumio Arai, Isao Kobayashi, and Daisuke Sugiyama viiHuman Trophectoderm Spheroid Derived from Human Embryonic Stem Cells. . . . . . . . . . . . 181 Wen Huang, Sze Wan Fong, William Shu Biu Yeung, and Yin Lau Lee Embryoid Bodies–Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions. . . . . . 189 Luciana Isaja, Sofı´a Luja´n Ferriol-Laffouillere, Sofı´a Mucci, Marı´a Soledad Rodrı´guez-Varela, and Leonardo Romorini A Simple, Rapid, and Cost-Effective Method for Loss-of-Function Assays in Pluripotent Cells. . . . . . . . . . 199 Helena Covelo-Molares, Yara Souto, Miguel Fidalgo, and Diana Guallar Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation. . . . . . . . . 215 Magdalena Guerra-Crespo, Omar Collazo-Navarrete, Rodrigo Ramos-Acevedo, Carmen Alejandra Morato-Torres, and Birgitt Schu¨le A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation . . . . . . . 233 Jianqun Zheng, Ying Ye, Qiushi Xu, Wei Xu, Wensheng Zhang, and Xi Chen Osteogenic Differentiation from Mouse Embryonic Stem Cells . . . . . . . 261 Zahra Alvandi and Michal Opas Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation . . . . 265 Manar Elkenani and Belal A. Mohamed Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions . . . . . . . . . . . . 275 Emre Balbasi, Gozde Guven, and Nihal Terzi Cizmecioglu Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages . . . . . . . . 295 Emre Balbasi, Dersu Sezginmert, Ceren Alganatay, and Nihal Terzi Cizmecioglu Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling . . . . . . . . . . . . . 309 Rubens Gomes-Ju´nior, Patrı´cia Shigunov, Bruno Dallagiovanna, and Isabela Tiemy Pereira Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors . . . . . . . . . . 321 Giulia Pavani, Joshua G. Klein, Deborah L. French, and Paul Gadue Proteomic Analysis of Human Neural Stem Cell Differentiation by SWATH-MS . . . . . . . . . . . . . 335 Jirina Tyleckova, Jakub Cervenka, Ievgeniia Poliakh, Jaromir Novak, Katerina Vodickova Kepkova, Helena Kupcova Skalnikova, and Petr Vodicka Index . . . . . . . . . . 361   مشخصات فایل
عنوان (Title):	Embryonic Stem Cell Protocols
نام فایل (File name):	929-www.GeneProtocols.ir-Embryonic Stem Cell Protocols-Humana (2022).pdf
عنوان فارسی (Title in Persian):	پروتکل های سلول بنیادی جنینی
ایجاد کننده:	Kursad Turksen
زبان (Language):	انگلیسی English
سال انتشار:	2022
شابک ISBN:	1071624369, 9781071624364
نوع سند (Doc. type):	کتاب
فرمت (File extention):	PDF
حجم فایل (File size):	13.8 مگابایت
تعداد صفحات (Book length in pages):	363

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